Journal: PLoS ONE

Article Title: RNA-Sequencing Analysis of HepG2 Cells Treated with Atorvastatin

doi: 10.1371/journal.pone.0105836

Figure Lengend Snippet: Top biological functions identified by Ingenuity Pathway Analysis affected by atorvastatin treatment.

Article Snippet: In the dose response experiments, cells were treated with water dissolved (3S, 5S)-atorvastatin sodium salt (Toronto Research Chemicals North York, Ontario, Canada) at various concentrations (0, 2.5, 5, 10, 20 and 40 µM) for 24 hours in four independent experiments.

Techniques:

Journal: PLoS ONE

Article Title: RNA-Sequencing Analysis of HepG2 Cells Treated with Atorvastatin

doi: 10.1371/journal.pone.0105836

Figure Lengend Snippet: Top five canonical pathways associated with the differentially expressed genes (n = 121) in atorvastatin treated HepG2 cells identified by Ingenuity Pathway Analysis.

Article Snippet: In the dose response experiments, cells were treated with water dissolved (3S, 5S)-atorvastatin sodium salt (Toronto Research Chemicals North York, Ontario, Canada) at various concentrations (0, 2.5, 5, 10, 20 and 40 µM) for 24 hours in four independent experiments.

Techniques:

Journal: PLoS ONE

Article Title: RNA-Sequencing Analysis of HepG2 Cells Treated with Atorvastatin

doi: 10.1371/journal.pone.0105836

Figure Lengend Snippet: (A) The canonical splice variant with accession number NM_138621 and the alternatively spliced variant NM_006538 of BCL2L11 are shown. Sixteen additional transcript variants of BCL2L11 are annotated in the National Center for Biotechnology Information (NCBI) Reference Sequence (RefSeq) database (not shown here). Exons are represented by blue boxes separated by intervening sequences (introns). (B) The CummeRbund expression bar plot is shown. FPKM, fragments per kilobase of transcript per million fragments mapped, reflects the mRNA expression level of NM_138621 and NM_006538 in the un-treated (Ctrl) and atorvastatin treated (Stat) HepG2 cells. The HepG2 samples showed low expression levels of the other sixteen alternatively spliced variants (FPKM<1). An asterisk indicates the statistically significant down-regulation of NM_138621 upon atorvastatin treatment after a Benjamini-Hochberg correction (5% FDR). The alternatively spliced variant NM_006538 was slightly upregulated.

Article Snippet: In the dose response experiments, cells were treated with water dissolved (3S, 5S)-atorvastatin sodium salt (Toronto Research Chemicals North York, Ontario, Canada) at various concentrations (0, 2.5, 5, 10, 20 and 40 µM) for 24 hours in four independent experiments.

Techniques: Variant Assay, Sequencing, Expressing